下载GeneBank文件(.gb)

LOCUS       pHANNIBAL.        5824 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for silencing genes in plants using intron-containing hairpin
            RNA (hpRNA).
ACCESSION   .
VERSION     .
KEYWORDS    pHANNIBAL.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding
            PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, 
            Green AG, Waterhouse PM.
  TITLE     Construct design for efficient, effective and high-throughput gene 
            silencing in plants.
  JOURNAL   Plant J. 2001;27:581-90.
  PUBMED    11576441
REFERENCE   2  (bases 1 to 5824)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2001"; volume: "27"; pages: "581-90"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..35)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(215..643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        670..774
                     /label=AmpR promoter
     CDS             775..1632
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1806..2394
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2682..2703
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2718..2748
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2756..2772
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2780..2796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2827..2845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3863..4208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4210..4228
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     intron          4236..5002
                     /label=PDK intron
                     /note="pyruvate orthophosphate dikinase intron from
                     Flaveria trinervia"
     misc_feature    5022..5045
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     terminator      5049..5755
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(5796..5814)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"