- 载体名称:
- pQE-100 DoubleTag
- 载体抗性:
- Ampicillin
- 载体长度:
- 3512 bp
- 载体类型:
- Qiagen Vectors
- 复制子:
- ori
- 载体来源:
- Qiagen
- 拷贝数:
- High copy number
pQE-100 DoubleTag 载体载体图谱
pQE-100 DoubleTag 载体载体序列
LOCUS pQE-100_DoubleTa 3512 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing proteins with an N-terminal 6xHis tag and a C-terminal Tag-100 tag. ACCESSION . VERSION . KEYWORDS pQE-100 DoubleTag. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3512) AUTHORS Qiagen TITLE Direct Submission REFERENCE 2 (bases 1 to 3512) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3512 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" CDS 115..117 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 127..144 /label=6xHis /note="6xHis affinity tag" misc_feature 145..184 /label=MCS /note="MCS" /note="multiple cloning site" CDS 193..228 /label=Tag-100 /note="epitope tag derived from MAP kinase 2" misc_feature 243..253 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" terminator 259..353 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 397..1053 /label=CmR /note="chloramphenicol acetyltransferase" terminator 1121..1207 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1362..1502 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(1688..2276) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2450..3307) /label=AmpR /note="beta-lactamase" promoter complement(3308..3412) /label=AmpR promoter
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