纽普生物

pQE-60载体

载体基本信息
载体名称 pQE-60 载体抗性 Ampicillin
载体长度 3431 bp 载体类型 Qiagen Vectors
载体来源 Qiagen 拷贝数 High copy number

pQE-60载体质粒图谱

pQE-60载体质粒图谱

pQE-60载体载体序列

LOCUS       Exported                3431 bp ds-DNA    circular SYN 05-12-2013
DEFINITION  Bacterial vector for expressing C-terminally 6xHis-tagged proteins, 
            with an NcoI site at the start codon.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-60
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     To use the start codon from a coding sequence, insert at NcoI.
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     misc_feature    113..132
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    157..167
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      173..267
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1039..1125
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1607..2195)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2366..3226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3227..3331)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatggga
      121 ggatccagat ctcatcacca tcaccatcac taagcttaat tagctgagct tggactcctg
      181 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
      241 cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa
      301 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      361 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      421 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      481 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      541 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      601 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      661 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      721 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      781 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgc atgggcaaat
      841 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
      901 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
      961 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
     1021 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1081 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagagc
     1141 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1261 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     1321 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     1381 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     1441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2041 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3361 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3421 ttcgtcttca c
//

pQE-60载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3431
常规描述Bacterial vector for expressing C-terminally 6xHis-tagged proteins, with an NcoI site at the start codon.
常规是否环形1
常规作者Qiagen
常规注释
来源实验宿主Escherichia coli1..3431
来源分子类型other DNA1..3431
来源种属synthetic DNA construct1..3431
启动子备注T5 promoter,bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator10..54
蛋白结合位点嵌合范围lac repressor encoded by lacI30..46
蛋白结合位点备注lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).30..46
蛋白结合位点嵌合范围lac repressor encoded by lacI62..78
蛋白结合位点备注lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).62..78
核糖体结合位点备注ribosome binding site101..106
其它特征区备注MCS,multiple cloning site113..132
编码区翻译起始位点1115..117
编码区备注ATG115..117
编码区产物start codon115..117
编码区翻译序列M115..117
编码区翻译起始位点1133..150
编码区备注6xHis133..150
编码区产物6xHis affinity tag133..150
编码区翻译序列HHHHHH133..150
其它特征区备注stop codons,stop codons in all three reading frames157..167
终止子基因 173..267
终止子备注lambda t0 terminator,transcription terminator from phage lambda173..267
终止子基因Escherichia coli rrnB1039..1125
终止子备注rrnB T1 terminator,transcription terminator T1 from the E. coli rrnB gene1039..1125
复制起点方向LEFT1607..2195
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication1607..2195
编码区翻译起始位点12366..3226
编码区基因bla2366..3226
编码区备注AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics2366..3226
编码区产物beta-lactamase2366..3226
编码区翻译序列MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW2366..3226
启动子基因bla3227..3331
启动子备注AmpR promoter3227..3331

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