载体基本信息 | |||
载体名称 | pB-RS | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5231 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | OriGene | 拷贝数 | High copy number |
pB-RS 载体载体图谱
pB-RS 载体载体序列
LOCUS pB-RS. 5231 bp DNA circular SYN 01-JAN-1980 DEFINITION HuSH-29 retroviral vector with ampicillin and blasticidin resistance markers, for expressing an shRNA from the U6 promoter. ACCESSION . VERSION . KEYWORDS pB-RS. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5231) AUTHORS OriGene TITLE Direct Submission REFERENCE 2 (bases 1 to 5231) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT The shRNA cassette consists of a 29-bp gene-specific sequence, followed by a 7-bp loop, followed by the reverse complement of the 29-bp sequence, followed by a TTTTTT terminator. FEATURES Location/Qualifiers source 1..5231 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 90..330 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 334..385 /label=shRNA site /note="shRNA site" /note="shRNA insertion site" promoter 441..651 /label=SV40 promoter /note="SV40 early promoter" CDS 673..1068 /label=BSD /note="blasticidin S deaminase" LTR 1150..1743 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" rep_origin complement(2115..2703) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2884..3741) /label=AmpR /note="beta-lactamase" promoter complement(3742..3846) /label=AmpR promoter LTR 3877..4346 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 4411..4610 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 4803..5219 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon"
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