载体基本信息 | |||
载体名称 | pBABE-Hygro | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5558 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Morgenstern JP, Land H. | 拷贝数 | High copy number |
pBABE-Hygro 载体载体图谱
pBABE-Hygro 载体载体序列
LOCUS pBABE-Hygro. 5558 bp DNA circular SYN 01-JAN-1980 DEFINITION Retroviral expression vector derived from the Moloney murine leukemia virus. Contains a hygromycin resistance marker. ACCESSION . VERSION . KEYWORDS pBABE-Hygro. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5558) AUTHORS Morgenstern JP, Land H. TITLE Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. JOURNAL Nucleic Acids Res. 1990;18:3587-96. PUBMED 2194165 REFERENCE 2 (bases 1 to 5558) AUTHORS Cell Biolabs TITLE Direct Submission REFERENCE 3 (bases 1 to 5558) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1990"; volume: "18"; pages: "3587-96" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT Strong expression is driven by the viral promoter in the 5' LTR. The commercially provided sequence was modified to include the splice donor mutation. FEATURES Location/Qualifiers source 1..5558 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 8..477 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 541 /label=splice donor mutation /note="splice donor mutation" /note="T-to-C mutation that blocks RNA splicing" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" misc_feature 1355..1402 /label=MCS /note="MCS" /note="multiple cloning site" promoter 1413..1742 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 1770..2792 /label=HygR /note="aminoglycoside phosphotransferase from E. coli" LTR 2839..3432 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" rep_origin complement(3804..4392) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4573..5430) /label=AmpR /note="beta-lactamase" promoter complement(5431..5535) /label=AmpR promoter
我们不提供该载体,载体信息仅供查询和参考。