pBABE-Zeo 载体 (V010489#)

载体基本信息
载体名称 pBABE-Zeo 载体抗性 Ampicillin
载体长度 4888 bp 载体类型 Viral Expression & Packaging Vectors
载体来源 Morgenstern JP, Land H. 拷贝数 High copy number

pBABE-Zeo 载体载体图谱

pBABE-Zeo4888 bp6001200180024003000360042004800LTR (truncated)MMLV Psigag (truncated)MCSSV40 promoterBleoRlong terminal repeat from Moloney murine leukemia virusoriAmpRAmpR promoter

pBABE-Zeo 载体载体序列

LOCUS       Exported                4888 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a Zeocin(TM) resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Zeo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR.
            
            The commercially provided sequence was modified to include the 
            splice donor mutation.
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     LTR             8..479
                     /note="LTR (truncated)"
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    541
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /note="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /product="
                     "
                     /note="gag (truncated)"
                     /note="truncated MMLV gag gene lacking the start codon"
     misc_feature    1355..1402
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1593..1728
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             1749..2123
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             2169..2762
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(3134..3722)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3900..4760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4761..4865)
                     /gene="bla"
                     /note="AmpR promoter"

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