载体基本信息 | |||
载体名称 | pBABE-Zeo | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4888 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Morgenstern JP, Land H. | 拷贝数 | High copy number |
pBABE-Zeo 载体载体图谱
pBABE-Zeo 载体载体序列
LOCUS Exported 4888 bp ds-DNA circular SYN 29-11-2013 DEFINITION Retroviral expression vector derived from the Moloney murine leukemia virus. Contains a Zeocin(TM) resistance marker. ACCESSION . VERSION . KEYWORDS pBABE-Zeo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4888) AUTHORS Morgenstern JP, Land H. TITLE Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. JOURNAL Nucleic Acids Res. 1990;18:3587-96. PUBMED 2194165 REFERENCE 2 (bases 1 to 4888) AUTHORS Cell Biolabs TITLE Direct Submission COMMENT Strong expression is driven by the viral promoter in the 5' LTR. The commercially provided sequence was modified to include the splice donor mutation. FEATURES Location/Qualifiers source 1..4888 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" LTR 8..479 /note="LTR (truncated)" /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 541 /note="splice donor mutation" /note="T-to-C mutation that blocks RNA splicing" misc_feature 542..741 /note="MMLV Psi" /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /product=" " /note="gag (truncated)" /note="truncated MMLV gag gene lacking the start codon" misc_feature 1355..1402 /note="MCS" /note="multiple cloning site" promoter 1413..1742 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 1593..1728 /note="SV40 ori" /note="SV40 origin of replication" CDS 1749..2123 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /note="BleoR" /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" LTR 2169..2762 /note="long terminal repeat from Moloney murine leukemia virus" rep_origin complement(3134..3722) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3900..4760) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4761..4865) /gene="bla" /note="AmpR promoter"
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