- 载体名称:
- pLenti3.3/TR
- 载体抗性:
- Ampicillin
- 载体长度:
- 9228 bp
- 载体类型:
- Viral Expression & Packaging Vectors
- 复制子:
- ori
- 载体来源:
- Invitrogen (Life Technologies)
- 筛选标记:
- Neomycin/G418(Geneticin)
- 拷贝数:
- High copy number
- 启动子:
- RSV
pLenti3.3/TR 载体载体图谱
pLenti3.3/TR 载体载体序列
LOCUS pLenti3.3_TR. 9228 bp DNA circular SYN 01-JAN-1980 DEFINITION Lentiviral vector for constitutive high-level expression of the Tet repressor in the T-REx(TM) system. ACCESSION . VERSION . KEYWORDS pLenti3.3 TR. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9228) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 9228) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9228 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 3..229 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 230..410 /label=5' LTR (truncated) /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 457..582 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1075..1308 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1686..1727 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 1804..1921 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 1944..2247 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2248..2451 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 2567..3139 /label=beta-globin intron /note="intron from rabbit beta-globin gene" promoter 3194..3212 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3223..3843 /label=TetR /note="tetracycline repressor TetR" misc_feature 3897..4485 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" promoter 4500..4816 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 4883..5674 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" LTR 5749..5982 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6054..6188 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 6215..6350 /label=SV40 ori /note="SV40 origin of replication" promoter complement(6371..6389) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6399..6415) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 6557..7012 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7038..7142 /label=AmpR promoter CDS 7143..8000 /label=AmpR /note="beta-lactamase" rep_origin 8174..8762 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 9050..9071 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 9086..9116 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9124..9140 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9148..9164 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 9185..9203 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase"
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