载体基本信息 | |||
载体名称 | pLenti6/TR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 8354 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pLenti6/TR 载体载体图谱
pLenti6/TR 载体载体序列
LOCUS pLenti6_TR. 8354 bp DNA circular SYN 01-JAN-1980 DEFINITION Lentiviral vector for high-level constitutive expression of the Tet repressor. ACCESSION . VERSION . KEYWORDS pLenti6 TR. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8354) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 8354) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8354 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 3..229 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 230..410 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 457..582 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1075..1308 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1686..1727 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" enhancer 1816..2119 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2120..2323 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 2552..3124 /label=beta-globin intron /note="intron from rabbit beta-globin gene" promoter 3179..3197 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3208..3828 /label=TetR /note="tetracycline repressor TetR" CDS 3870..3899 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" promoter 3947..4276 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 4324..4371 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 4390..4785 /label=BSD /note="blasticidin S deaminase" LTR 4875..5108 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5180..5314 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 5341..5476 /label=SV40 ori /note="SV40 origin of replication" promoter complement(5497..5515) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5525..5541) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5683..6138 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6164..6268 /label=AmpR promoter CDS 6269..7126 /label=AmpR /note="beta-lactamase" rep_origin 7300..7888 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8176..8197 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 8212..8242 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8250..8266 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8274..8290 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 8311..8329 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase"
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