pLenti6/TR 载体 (V010461#)

载体基本信息
载体名称 pLenti6/TR 载体抗性 Ampicillin
载体长度 8354 bp 载体类型 Viral Expression & Packaging Vectors
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number

pLenti6/TR 载体载体图谱

pLenti6/TR8354 bp400800120016002000240028003200360040004400480052005600600064006800720076008000RSV promoter5' LTR (truncated)HIV-1 PsiRREgp41 peptideProtein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509CMV enhancerCMV promoterbeta-globin intronT7 promoterTetRMycSV40 promoterEM7 promoterBSD3' LTR (Delta-U3)SV40 poly(A) signalSV40 oriT7 promoterM13 fwdf1 oriAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 revT3 promoter

pLenti6/TR 载体载体序列

LOCUS       pLenti6_TR.        8354 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector for high-level constitutive expression of the Tet 
            repressor.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti6 TR.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2552..3124
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        3179..3197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3208..3828
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             3870..3899
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     promoter        3947..4276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4324..4371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4390..4785
                     /label=BSD
                     /note="blasticidin S deaminase"
     LTR             4875..5108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5180..5314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5341..5476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5497..5515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5525..5541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5683..6138
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6164..6268
                     /label=AmpR promoter
     CDS             6269..7126
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7300..7888
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8176..8197
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8212..8242
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8266
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8311..8329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"

我们不提供该载体,载体信息仅供查询和参考。