Yeast three-hybrid vector for expressing a "bait" protein fused to the GAL4 DNA-binding domain, plus an additional bridge protein.
- 载体名称:
- pBridge
- 载体抗性:
- Ampicillin
- 载体长度:
- 6527 bp
- 载体类型:
- Yeast Plasmids
- 复制子:
- ori
- 载体来源:
- Clontech
- 拷贝数:
- High copy number
- 启动子:
- ADH1
pBridge 载体载体图谱
pBridge 载体载体序列
LOCUS pBridge. 6527 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast three-hybrid vector for expressing a """"bait"""" protein fused to the GAL4 DNA-binding domain, plus an additional bridge protein. ACCESSION . VERSION . KEYWORDS pBridge. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6527) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 6527) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6527 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..406 /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 434..874 /label=GAL4 DNA binding domain /note="DNA binding domain of the GAL4 transcriptional activator" misc_feature 878..905 /label=MCS I /note="MCS 1" /note="multiple cloning site" terminator 921..1108 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(1164..1835) /label=TRP1 /note="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" promoter complement(1836..1937) /label=TRP1 promoter primer_bind complement(1951..1967) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1975..1991) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1999..2029) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2044..2065) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2259..2609 /label=MET17 promoter /note="expression is repressed by methionine" CDS 2619..2621 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 2631..2657 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" CDS 2679..2699 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" misc_feature 2700..2723 /label=MCS II /note="MCS 2" /note="multiple cloning site" terminator 2825..3095 /label=PGK1 terminator /note="transcription terminator for 3-phosphoglycerate kinase gene" rep_origin complement(3357..3945) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4119..4976) /label=AmpR /note="beta-lactamase" promoter complement(4977..5081) /label=AmpR promoter rep_origin 5363..6527 /label=2u ori /note="yeast 2u plasmid origin of replication"
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