纽普生物

pESC-HIS载体

载体基本信息
载体名称 pESC-HIS 载体抗性 Ampicillin
载体长度 6706 bp 载体类型 Yeast Plasmids
载体来源 Agilent Technologies 拷贝数 High copy number

pESC-HIS载体质粒图谱

pESC-HIS载体质粒图谱

pESC-HIS载体载体序列

LOCUS       Exported                6706 bp ds-DNA    circular SYN 31-5-2016
DEFINITION  Yeast episomal vector with a HIS3 marker, for galactose-regulated 
            expression and tagging of up to two genes.
ACCESSION   AF063850
VERSION     .
KEYWORDS    pESC-HIS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        317..503
                     /gene="S. cerevisiae HIS3"
                     /note="HIS3 promoter"
     CDS             504..1163
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /note="HIS3"
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     terminator      1961..2126
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    2256..2339
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     CDS             complement(2274..2297)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     promoter        2344..3008
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /note="GAL1,10 promoter"
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    2560..2677
                     /bound_moiety="Gal4"
                     /note="UAS"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    3012..3088
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     CDS             3055..3084
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     terminator      3114..3303
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3546..4134)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4305..5165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5166..5270)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      5297..6639
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc
     1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa
     2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata
     2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg
     2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
     2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg
     2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg
     2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
     2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat
     2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt
     2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg
     2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
     2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
     2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
     2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
     2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa
     2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
     2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
     3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag
     3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct
     3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
     3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
     3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
     3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc
     6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//

pESC-HIS载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度6706
常规描述Yeast episomal vector with a HIS3 marker, for galactose-regulated expression and tagging of up to two genes.
常规是否环形1
常规作者Agilent Technologies
常规注释
来源实验宿主Saccharomyces cerevisiae1..6706
来源分子类型other DNA1..6706
来源种属synthetic DNA construct1..6706
启动子基因S. cerevisiae HIS3317..503
启动子备注HIS3 promoter317..503
编码区翻译起始位点1504..1163
编码区基因S. cerevisiae HIS3504..1163
编码区备注HIS3,yeast auxotrophic marker504..1163
编码区产物imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis504..1163
编码区翻译序列MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAEQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAFKEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM504..1163
复制起点方向LEFT1423..1878
复制起点备注f1 ori,f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis1423..1878
终止子基因S. cerevisiae ADH11961..2126
终止子备注ADH1 terminator,transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene1961..2126
其它特征区备注MCS 1,multiple cloning site 12256..2339
编码区翻译起始位点12274..2297
编码区备注FLAG2274..2297
编码区产物FLAG(R) epitope tag, followed by an enterokinase cleavage site2274..2297
编码区翻译序列DYKDDDDK2274..2297
启动子基因GAL1 and GAL10 genes of S. cerevisiae2344..3008
启动子备注GAL1,10 promoter,divergent inducible promoter, regulated by Gal42344..3008
蛋白结合位点嵌合范围Gal42560..2677
蛋白结合位点备注UAS,upstream activating sequence mediating Gal4-dependent induction2560..2677
其它特征区备注MCS 2,multiple cloning site 23012..3088
编码区翻译起始位点13055..3084
编码区备注Myc3055..3084
编码区产物Myc (human c-Myc oncogene) epitope tag3055..3084
编码区翻译序列EQKLISEEDL3055..3084
终止子基因S. cerevisiae CYC13114..3303
终止子备注CYC1 terminator,transcription terminator for CYC13114..3303
复制起点方向LEFT3546..4134
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication3546..4134
编码区翻译起始位点14305..5165
编码区基因bla4305..5165
编码区备注AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics4305..5165
编码区产物beta-lactamase4305..5165
编码区翻译序列MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW4305..5165
启动子基因bla5166..5270
启动子备注AmpR promoter5166..5270
复制起点备注2u ori,yeast 2u plasmid origin of replication5297..6639

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