- 载体名称:
- pPIC9K
- 载体抗性:
- Ampicillin
- 载体长度:
- 9276 bp
- 载体类型:
- Yeast Plasmids
- 复制子:
- ori
- 载体来源:
- Invitrogen (Life Technologies)
- 拷贝数:
- High copy number
- 启动子:
- AOX1
pPIC9K 载体载体图谱
pPIC9K 载体载体序列
LOCUS pPIC9K. 9276 bp DNA circular SYN 01-JAN-1980 DEFINITION Pichia pastoris multi-copy integrating vector for methanol-inducible expression of a secreted protein. ACCESSION . VERSION . KEYWORDS pPIC9K. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9276) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 9276) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9276 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..937 /label=AOX1 promoter /note="inducible promoter, regulated by methanol" CDS 949..1215 /label=alpha-factor secretion signal /note="N-terminal secretion signal from S. cerevisiae alpha-factor" misc_feature 1216..1241 /label=MCS /note="MCS" /note="multiple cloning site" terminator 1321..1567 /label=AOX1 terminator /note="transcription terminator for AOX1" CDS complement(1980..4514) /codon_start=1 /gene="Pichia pastoris HIS4" /product="multifunctional enzyme, required for histidine biosynthesis" /label=Pichia pastoris HIS4 /note="PpHIS4" /note="auxotrophic marker for Pichia pastoris" /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI" CDS complement(4931..5743) /label=KanR /note="aminoglycoside phosphotransferase" misc_feature 6122..6878 /label=AOX1 3' fragment /note="region downstream of Pichia pastoris AOX1 gene" misc_feature 7021..7161 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(7347..7935) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8109..8966) /label=AmpR /note="beta-lactamase" promoter complement(8967..9071) /label=AmpR promoter
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