pPICZ B 载体图谱和序列

载体名称:: pPICZ B

载体抗性:: Bleomycin

载体长度:: 3328 bp

载体类型:: Yeast Plasmids

复制子:: ori

载体来源:: Invitrogen (Life Technologies)

拷贝数:: High copy number

启动子:: AOX1

pPICZ B 载体载体图谱

pPICZ B 载体载体序列

Copy Sequence

下载GeneBank文件(.gb)

LOCUS       40924_34575        3328 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3328)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             1010..1039
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1055..1072
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1152..1398
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1413..1824
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1832..1879
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1898..2269
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2338..2585
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2660..3248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

我们不提供该载体，载体信息仅供查询和参考。