pPICZα B 载体图谱和序列

pPICZα B vector (V010240) is a Pichia pastoris vector for methanol-inducible expression of a secreted protein. There are other similar expression vectors, such as pPICZα A or pPICZα C For other reading frames.

载体基本信息
载体名称	pPICZα B	载体抗性	Bleomycin
载体长度	3597 bp	载体类型	Yeast Plasmids
载体来源	Invitrogen (Life Technologies)	拷贝数	High copy number

pPICZα B 载体载体图谱

pPICZα B 载体载体序列

Copy Sequence

下载GeneBank文件(.gb)

LOCUS       pPICZ-B.        3597 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pPICZ-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3597)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1206..1280
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1279..1308
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1324..1341
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1421..1667
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1682..2093
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2101..2148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2167..2538
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2607..2854
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

我们不提供该载体，载体信息仅供查询和参考。