纽普生物

pPICZα C载体

载体基本信息
载体名称 pPICZα C 载体抗性 Bleomycin
载体长度 3598 bp 载体类型 Yeast Plasmids
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number

pPICZα C载体质粒图谱

pPICZα C载体质粒图谱

pPICZα C载体载体序列

LOCUS       Exported                3598 bp ds-DNA    circular SYN 05-2-2013
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pPICZ(alpha) C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/3/1 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="synthetic DNA construct"
                     /lab_host="Pichia pastoris"
                     /mol_type="other DNA"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /note="alpha-factor secretion signal"
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     misc_feature    1207..1281
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1280..1309
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             1325..1342
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      1422..1668
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        1708..2094
                     /gene="S. cerevisiae TEF1"
                     /note="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        2102..2149
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             2168..2542
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2608..2855
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2930..3518)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga agagagaggc
     1201 tgaagcatcg atgaattcac gtggcccagc cggccgtctc ggatcggtac ctcgagccgc
     1261 ggcggccgcc agctttctag aacaaaaact catctcagaa gaggatctga atagcgccgt
     1321 cgaccatcat catcatcatc attgagtttg tagccttaga catgactgtt cctcagttca
     1381 agttgggcac ttacgagaag accggtcttg ctagattcta atcaagagga tgtcagaatg
     1441 ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta acctatatag
     1501 tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg atcagcctat
     1561 ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag tttgatgttt
     1621 ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagacc ttcgtttgtg
     1681 cggatccccc acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt
     1741 ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt
     1801 ttccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa
     1861 aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt
     1921 ttctttttct tgaaattttt ttttttagtt tttttctctt tcagtgacct ccattgatat
     1981 ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta
     2041 caactttttt tacttcttgt tcattagaaa gaaagcatag caatctaatc taaggggcgg
     2101 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2161 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2221 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2281 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2341 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     2401 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     2461 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     2521 cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca ggcctcggag
     2581 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
     2641 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     2701 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     2761 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     2821 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa
     2881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3121 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3361 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagatc
//

pPICZα C载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3598
常规描述Pichia pastoris vector for methanol-inducible expression of a secreted protein.
常规是否环形1
常规作者Invitrogen (Life Technologies)
常规注释
来源实验宿主Pichia pastoris1..3598
来源分子类型other DNA1..3598
来源种属synthetic DNA construct1..3598
启动子基因Pichia pastoris AOX12..940
启动子备注AOX1 promoter,inducible promoter, regulated by methanol2..940
编码区翻译起始位点1941..1207
编码区基因MF-alpha-1941..1207
编码区备注alpha-factor secretion signal,Cleavage by the Kex2 protease occurs after the dibasic KR sequence. The EA dipeptides are then removed by dipeptidyl aminopeptidase A.941..1207
编码区产物N-terminal secretion signal from S. cerevisiae alpha-factor941..1207
编码区翻译序列MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA941..1207
其它特征区备注MCS,multiple cloning site1207..1281
编码区翻译起始位点11280..1309
编码区备注Myc1280..1309
编码区产物Myc (human c-Myc oncogene) epitope tag1280..1309
编码区翻译序列EQKLISEEDL1280..1309
编码区翻译起始位点11325..1342
编码区备注6xHis1325..1342
编码区产物6xHis affinity tag1325..1342
编码区翻译序列HHHHHH1325..1342
终止子基因Pichia pastoris AOX11422..1668
终止子备注AOX1 terminator,transcription terminator for AOX11422..1668
启动子基因S. cerevisiae TEF11708..2094
启动子备注TEF1 promoter,promoter for EF-1-alpha1708..2094
启动子备注EM7 promoter,synthetic bacterial promoter 2102..2149
编码区翻译起始位点12168..2542
编码区基因Sh ble from Streptoalloteichus hindustanus2168..2542
编码区备注BleoR,confers resistance to bleomycin, phleomycin, and Zeocin(TM)2168..2542
编码区产物antibiotic-binding protein2168..2542
编码区翻译序列MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD2168..2542
终止子基因S. cerevisiae CYC12608..2855
终止子备注CYC1 terminator,transcription terminator for CYC12608..2855
复制起点方向LEFT2930..3518
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication2930..3518

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